Troubleshooting in immunohistochemistry (IHC) can be highly complex and is more than "what's wrong with or why didn't my slides stain?" There are many issues affecting IHC quality and the ability of technical staff to troubleshoot. Some issues related to IHC problems are: deviating from established IHC protocols, no standardization of IHC protocols, introducing multiple variables in the IHC process, antigen retrieval optimization, improper antigen retrieval techniques and use of heat sources.
Art and Science of Laboratory Medicine
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